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BACTERIAL DnaB HELICASE SEQUENCE ANALYSIS
This is a list of the first 22 bacteria whose DnaB helicase sequences were determined. The genes are called dnaB unless otherwise indicated.
- Aquifex aeolicus, Aquificales
- Bacillus mojavensis dnaC gene, Firmicutes Clostridium
- Bacillus stearothermophilus; Firmicutes
- Bacillus subtilis dnaC gene, Firmicutes Low G+C gram-positive
- Borrelia burgdorferi, Spirochaetales
- Chlamydia psittaci, Chlamydiales
- Chlamydia trachomatis, Chlamydiales
- Escherichia coli, Proteobacteria gamma
- Haemophilus influenzae, Proteobacteria gamma
- Helicobacter pylori, Proteobacteria epsilon
- Mycobacterium leprae, Firmicutes Actinobacteria
- Mycobacterium tuberculosis, Firmicutes Actinomycetes
- Mycoplasma genitalium, Firmicutes Low G+C gram-positive
- Mycoplasma pneumoniae, Firmicutes Low G+C gram-positive
- Rhodothermus marinus,Cytophagales
- Rickettsia prowazekii, Proteobacteria alpha
- Salmonella typhimurium, Proteobacteria gamma
- Streptomyces coelicolor, Firmicutes Actinobacteria
- Synechocystis PCC6803, Cyanobacteria Chroococcales
- Thermotoga maritima, Thermotogales
- Thermus aquaticus, Thermus/Deinococcus group
- Treponema pallidum, Spirochaetales
The fork helicases were aligned using the Clustal V program using its default settings on June 16, 1999.
The sequences from M. tuberculosis, M. leprae, R. marinus, and Synechocystis each have an "X" in place of inteins to aid the alignment. The inteins are aligned on another page.
The following sequences are arranged in order of relatedness to that from E. coli. The symbols used to indicate the level of conservation are * = identical and . = highly conserved.
The secondary structures within the N-terminal domain of E. coli DnaB were determined by high resolution NMR methods by Weigelt et al. (1998, J. Biomol. NMR 11, 233) and x-ray crystallography by Fass et al. (1999 Structure 7, 691). The secondary structures are represented above the sequences. The abbreviations are "h" for alpha helix and "3" for 3sub10 helix. Each helix is also numbered from 1 to 7.
ECOLI MAGNKPF------------------NKQQAE----PRERDPQV-------
STYPH MAGNKPF------------------NKQQAE----PRERDPQV-------
HINFL MDFSTVFYFLFSPHFSLKSNRYLRYHRRHSSIEISPMASQPQIKSSDKKT
BSUBT MTD-----LLN---------------------------------------
BMOJA --------------------------------------------------
BSTEA MSE-----LFS---------------------------------------
TAQUA M---------E---------------------------------------
RMARI MAE-----FEERPRLSIGEEEAPPYPLEK--LTGGRRRTRAQIHALHQQA
TMARI M-------------------------------------------------
SYNEC3 MA--------ANPAL-----------------------------------
MLEPR MAVVDD---LAHSGMDAVPP------------------------------
MTUBE MAVVDD---LA-PGMDSSPP------------------------------
SCOEL MSISEP---LDDPWADSGPSDRLPASRRRTEGGRGRDEQHERGPDSGWDG
RPROW M-----------------------------------------VRNKINND
CTRAC2 MA------------------------------TQTKKPQPTQLLSL----
AAEOL M-------------------------------------------------
BBURG MALAA-------------------------------------------FS
TPALL MPGMP-------------------------------------------NP
HPYLO MDHLKHLQQLQN--------------------------------------
CPSIT M-------------------------IKQSEEEK----------------
CTRAC1 M-------------------------KTRSEIEN----------------
MGENI M-------------------------------------------------
MPNEU M-------------VSNKLEQTFKFANDKNIER-----------------
secondary hhhhhh1hhhhhh hh2hh 333 hhhhh
ECOLI AGL---KVPPHSIEAEQSVLGGLMLDNERWDDVAERV-VADDFYTRPHRH
STYPH AGL---KVPPHSIEAEQSVLGGLMLDNERWDDVAERV-VADDFYTRPHRH
HINFL AQV---SIPPHSTEAEQAVLGGIMLSNQHWDGIAERV-IADDFYTFQHRL
BSUBT D-----RLPPQNIEAEQAVLGAIFLQPSALTLASEVL-IPDDFYRMSHQK
BMOJA ----------------------IFLQPSALTLASEVL-IPDDFYRMSHQK
BSTEA E-----RIPPQSIEAEQAVLGAVFLDPAALVPASEIL-IPEDFYRAAHQK
TAQUA G-----PIPPHSLEAEQSVLGSILLDSDVMDEVEGLLPSPEAFYAEAHRK
RMARI G-----RVPPQAVELEQAVLGAMLIEPEAIPRALEIL-TPEAFYDGRHQR
TMARI ------RVPPHNLEAEVAVLGSILIDPSVINDVLEILS-HEDFYLKKHQH
SYNEC3 --------PPQNIEAEECILGGILLDPEAMGRIIDLL-VVDAFYVKAHRL
MLEPR -SEDFGRQPPQDLAAEQSVLGGMLLSKDAIADVLERLR-SGDFYRPAHQN
MTUBE -SEDYGRQPPQDLAAEQSVLGGMLLSKDAIADVLERLR-PGDFYRPAHQN
SCOEL GGAAFERVPPQDLDAEQSVLGGMLLSKDAIADVVEILK-GHDFYKPAHET
RPROW DNLSIPRVLPSNIQAEQMLLGAIITNNALLSYVSEFLR-NEHFFEPIHQK
CTRAC2 ---------PNSKESEMIVLGCMLTSVNHLNLAANLLQ-EDDFYFLEHRI
AAEOL ---QFVDKLPCDESAERAVLGSMLEDPENIPLVLEYLK-EEDFCIDEHKL
BBURG NSALL-----FNDGAEKAVISSIFYNSDKIEQIAFHVR-ADDFYNETHKL
TPALL TQELKGKIPPHNLEAERAVLGAVLLDDSALSTATEQLS-ASSFYSAAHQR
HPYLO --------------IERIVLSGIVLANHKIEEVHSVLE-PSDFYYPPNGL
CPSIT ----------DLLDVEFFVLGQAVNYLEHAHVVVRRLS-EHHFKSENHKN
CTRAC1 ----------RMQDIEYALLGKALIFEDSTEYILRQLA-NYEFKCSHHKN
MGENI --------------------QAVAQNSEGIDLIFNKLE-PIDFFATPFKL
MPNEU --------------AERVLMQALVQDAVGVDLILTKLE-PKDFFGFPFNF
. *
secondary hh3hhhhhhhh hhhh----h4hhhh h5h hh6h
ECOLI IFTEMARLQES-GSPIDLITL----AESLERQGQL---DSVGGFAYLAEL
STYPH IFTEMARLQES-GSPIDLITL----AESLERQGQL---DSVGGFAYLAEL
HINFL IFTEMEHLMRN-QSPIDLITL----DQALRSRGVS---DEVGGFAYLAEL
BSUBT IYNAMLVLGDR-GEPVDLVTV----TSELANTDLL---EEVGGISYLTDI
BMOJA IYNAMLVLGDR-GEPVDLVTV----TSELANTDLL---EEVGGISYLTDI
BSTEA IFHAMLRVADR-GEPVDLVTV----TAELAASEQL---EEIGGVSYLSEL
TAQUA IYAAMQALRSQ-GRPVDLVTL----SEELSRRGQL---EEVGGTAYLLQL
RMARI IFRAIVRLFEQ-NRGVDLLTV----TEELRRTGEL---EQAGDTIYLSEL
TMARI IFRAMEELYDE-GKPVDVVSV----CDKLQSMGKL---EEVGGDLEVAQL
SYNEC3 IYEAMLSLHGQ-SQPTDLMSV----SSWLQDHHHF---EAIGGMVKLTQL
MLEPR VYDAILDLYGR-GEPADAVTV----AAELDRRGLL---RRIGGAPYLHTL
MTUBE VYDAILDLYGR-GEPADAVTV----AAELDRRGLL---RRIGGAPYLHTL
SCOEL VYQAILDVYAK-GEPADPITI----AAELTKRGEI---NKVGGASYLHTL
RPROW IYDAIEKIIEK-GLIATPITL----RSMLTQDALF---KEIEGEGYLAKL
CTRAC2 IFRVLQDAFKS-DRPMDPHLT----GEELKRRDQL---NIIGGPSYLITL
AAEOL LFRVLTNLWSEYGNKLDFVLI----KDHLEKKNLL---QKIP-IDWLEEL
BBURG IFKAMVSLYEK-RENIDPITVFEEVSKHVSKTQLLIKKDLINLPDYLDSL
TPALL IFQALVELSDL-GQRPDILVL----SEHLRSCEAL---DFVGGSAYVASL
HPYLO FFEIALKLHEE-DCPID-----ENFIRQKMPKDKQIKEED------LVAI
CPSIT IFLLIRDILRDRD-TISISLIWEEIKRRNLDKSM--------DVSYLIHM
CTRAC1 IFIVFK-YLKDNGLPITVDSAWEELLRRRI-KDM--------DKSYLGLM
MGENI IFQTAKENYQL-NNPIIGSGLLEAVKFKLDANDQSTKSELEI--LFTKIL
MPNEU IFQTAQENYSE-GNKLFGSALLEAVKFKLDT-DSKTQREIEG--LFEDVL
. .
secondary hhh hhhhh7h--hh
ECOLI SKNTPSAANISAYADI--VRERAV---VREMISVANEIAEAGFD-PQGRT
STYPH SKNTPSAANISAYADI--VRERAV---VRDMIAVAHEIADAGYD-PQGRN
HINFL SNNTPNAINILAYADI--VREKAI---LRELISVGNRIAENSYS-PKGQD
BSUBT ANSVPTAANIEYYAKI--VEEKSI---LRRLIRTATTIAQDGYT--REDE
BMOJA ANSVPTAANIEYYAKI--VEEKSI---LRRLIRTATTIAQDGYT--REDE
BSTEA ADAVPTAANVEYYARI--VEEKSV---LRRLIRTATSIAQDGYT--REDE
TAQUA SEATPTAAYAEHYARI--VAEKWT---LRRLIQAAGEAMRLAYE--EAGS
RMARI TTRVASAANVEYHARI--IAEKAL---LRRMIEVMTLLVGRAYD--PAAD
TMARI AEAVPSSAHALHYAEI--VKEKSI---LRKLIEISRKISESAY---MEED
SYNEC3 LDRTISAVNIDRFAAL--IMDKYL---RRQLIAAGHDIVDLGYE--TSKE
MLEPR ISTVPTAANAGYYAGI--VAEKAL---LRRLVEAGTRVVQYGYAGAEGAD
MTUBE ISTVPTAANAGYYASI--VAEKAL---LRRLVEAGTRVVQYGYAGAEGAD
SCOEL VQTVPTAANAAYYAEI--VHERAV---LRRLVEAGTRITQMGYAADD--D
RPROW ITMSMMVINPIDYGKI--IYDLAI---KRNLINIGEEVVNNAYNSSLAVA
CTRAC2 SEFAGTSAYIEEYAEI--IRSKSI---LRKMIQAAKDIEKKA--AEEPRD
AAEOL YEEAVSPDTLEEVCKI--VKQRSA---QRAIIQLGIELIHKG---KENKD
BBURG SVYLPTDRTINVYAKI--VKEQSV---RRSILNVSKELNDYINDSTK--S
TPALL TDAVPSAANVEYYTRI--VCDAAM---RRSLLKVARIITAEAFNDTV--S
HPYLO FAASPID-NIEAYVEE--IKNASI---KRKLFGLANTIREQALESAQKS-
CPSIT SQNADIPIDLDH--HIDFLHEKHV---NNLLKEFLDSSFQDFTRYPNRRS
CTRAC1 LHDALSNDKLRSVSHTVFLDDLSVCSAEENLSNFIFRSFNEYNENPLRRS
MGENI LIRLPPNQT----EIKTLVDVVKKASIFRRLQQFAKRVYNEEFKLKEDR-
MPNEU LKGLPFLER----DIKVFVDVVKKASIFRQLKQFAKKVEKEEFKVKDDR-
. .
ECOLI SEDLLDLAESRVFKIAESRANKDEGPKN--IADVLDA-------TVARIE
STYPH SDELLDLAESRVFQIAENRANKDEGPKS--IDQILDA-------TVARIE
HINFL IKLILDEAEREVFAIAEKRTTSSEGPQN--VINVLES-------TIEKID
BSUBT -EDLLSEAEK-IMEVAQRKNTS--AFQN--IKDVLVQ-------TYDNIE
BMOJA VEDLLSEAEKTIMEVAQRKNTS--AFQN--IKDVLVQ-------TYDNIE
BSTEA IDVLLDEADRKIMEVSQRKHSG--AFKN--IKDILVQ-------TYDNIE
TAQUA LDEILDTAGKKILEVALTKTDT--EARP--MRELVHE-------TFEHIE
RMARI AFELLDEVEAEIFRLSDVHLRK--AARS--MNEVVKE-------TLERLE
TMARI VEILLDNAEKMIFEISEMKTTK--SYDH--LRGIMHR-------VFENLE
SYNEC3 LETIFDESEQKIFRLTQSRPQA--GLVP--LSETLVN-------TFIELD
MLEPR VAEVVDRAQAEIYDVAECRLS--ENYVP--LEDLLQP-------TMDDLD
MTUBE VAEVVDRAQAEIYDVADRRLS--EDFVA--LEDLLQP-------TMDEID
SCOEL VDEIVNRAQAEIYAVTEQRTS--EDYLP--LGDIMEG-------ALDEIE
RPROW AKEQIEYAEAKLYDLTKEGLN-EKSFTK--VGISISE-------SLASIN
CTRAC2 VTTALDDAQNLLFRISQTTNLAPHVLVADKLKGVASSKDKSFLLALQERQ
AAEOL FHTLIEEAQSRIFSIAESATSTQFYHVKDVAEEVIE-----LIYKFKSSD
BBURG INEIVEESQQKILSI-ELDYSSKNLYHAKVIAERVHNEI--YERSMKKKE
TPALL GNIVLETAQREIYDL-T---NARRVATFKLLKNLIPDLVNTIETRYRNQS
HPYLO -SDILGAVEREVYALLNGSTIEGFRNIKEVLESAMDLITEN---------
CPSIT PYTLIDQFKERLDDIYEKTSYPRRKHIGKTVYDIFSSGENGKASVIAQIR
CTRAC1 PFLLLERIKGRLDSAIAKT-FSIRSARGRSIYDIFSQSEIG---VLARIK
MGENI FEGYLQAIQDDFVKIIHSAFSNIFAF--------------SYDEIANQEE
MPNEU FEGYLQAIQNDFTKIIHSAFVSIPGL--------------SYDEIVREEE
ECOLI QLFQ-----QPHDGVTGVNTGYDDLNKKTAGLQPSDLIIVAARPSMGKTT
STYPH QLFQ-----QPHDGVTGVDTGYQDLNKKTAGLQRSDLIIVAARPSMGKTT
HINFL ILSK----LENHSGVTGVTTGFTDLDKKTAGLQPSDLIIVAARPSMGKTT
BSUBT QLYNRKGDI------TGIPTGFTELDRMTAGFQRNDLIIVAARPSVGKTA
BMOJA QLHNRKGDI------TGIPTGFSELDRMTAGFQRNDLIIVAARPSVGKTA
BSTEA MLHNRDGEI------TGIPTGFTELDRMTSGFQRSDLIIVAARPSVGKTA
TAQUA ALFQNKGEV------AGVRTGFKELDQLIGTLGPGSLNIIAARPAMGKTA
RMARI AIHGRPGGI------TGVPSGFHQLDALTGGWQRGDLIIIAARPSMGKTA
TMARI NFRERANLIEPGVLITGLPTGFKSLDKQTTGFHSSDLVIIAARPSMGKTS
SYNEC3 KLHEKLSS-------PGVETQFYDLDAMTGGLQRADLIILAGRPSMGKTA
MLEPR AIASNGGI------SRGAPTGFTELDEVTNGLHPGQMIIVAARPGVGKXS
MTUBE AIASSGGL------ARGVATGFTELDEVTNGLHPGQMVIVAARPGVGKS-
SCOEL AIGSRSGE------MTGVPTGFTDFDSLTNGLHPGQMVVIAARPAMGKS-
RPROW R------AMKNNDHVIGISTGLLDLDNKLFGFHNSDLIILAGRPSMGKTA
CTRAC2 EAFQASAHDSSSPMLSGFPTHFLDLDKMISGFSPSNLIILAARPAMGKTA
AAEOL RL------------VTGLPSGFTELDLKTTGFHPGDLIILAARPGMGKTA
BBURG ANF-------------GIPSGFRKVDSLIGGFRNSDFIIVGARPSIGKTA
TPALL DLV-------------GIATGLTALDNLTGGFQNSELIVIGARPSMGKTA
HPYLO ---QRKGSLE----VTGIPTGFVQLDNYTSGFNKGSLVIIGARPSMGKTS
CPSIT HRYDYRSR-HKIDYVDGLPTGYSSIDEHSIILSRGNFVVIAARPAMGKTA
CTRAC1 KRRAAFSE-NQNSFFDGFPTGYKDIDDKGVILAKGNFVIIAARPSIGKTA
MGENI ALIKK--VHRGELIISGLSSGFLKLDQLTSGWKPGELIVIAARPGRGKTA
MPNEU ELIPK--VYRGELTVKGLKSGFYPLDQLTSGWKEGELIVVAARPGRGKTA
* . . ....**. **
ECOLI -FAMNLVENAAMLQ--DK--------PVLIFSLEMPSEQIMMRSLASLSR
STYPH -FAMNLCENAAMLQ--DK--------PVLIFSLEMPGEQIMMRMLASLSR
HINFL -FAMNLCENAAMAS--EK--------PVLVFSLEMPAEQIMMRMIASLAR
BSUBT -FALNIAQNVATKT--DE--------SVAIFSLEMGAEQLVMRMLCAEGN
BMOJA -FALNIAQNVATKT--DE--------SVAIFSLEMGAEQLVMRMLCAEGN
BSTEA -FALNIAQNVATKT--NE--------NVAIFSLEMSAQQLVMRMLCAEGN
TAQUA -FALTIAQNAALKE--GV--------GVGIYSLEMPAAQLTLRMMCSEAR
RMARI -FALSCARNAALHPHYGT--------GVAIFSLEMGAEQLAQRLLTAEAR
TMARI -FALSIARNMAVN--FEI--------PVGIFSLEMSKEQLAQRLLSMESG
SYNEC3 -FGLGIAANIAKNQNL----------PVAIFSLEMSKEQLALRLVASESL
MLEPR TLGLDFMRSCSIKHRLAS----------VIFSLEMSKSEIVMRLLSAEAK
MTUBE TLGLDFMRSCSIRHRMAS----------VIFSLEMSKSEIVMRLLSAEAK
SCOEL TLALDFARAASIKNNLPS----------VIFSLEMGRNEIAMRLLSAEAR
RPROW -FAINLALNTCNNMRLKNIRDNQEIKSVGFFSLEMSSEQLTTRLLSLCAE
CTRAC2 -LALNIVENFCFDSRL----------PVGIFSLEMTVDQLIHRIICSRSE
AAEOL -FMLSIIYNLA----------KDEGKPSAVFSLEMSKEQLVMRLLSMMSE
BBURG -FALNIASYIALR--------KEEKKKVGFFSLEMTADALIKRIISSQSC
TPALL -LAMTMASNIAIR----------QRIPTAFFSLEMSNLLLMQRLIAAESG
HPYLO -LMMNMV-----------LSALNDDRGVAVFSLEMSAEQLALRALSDLTS
CPSIT -FAIDIALNLVL----------EQGKAVGFISLEMSPNQIVERIISNLSE
CTRAC1 -LAIDMAINLAV----------TQQRRVGFLSLEMSAGQIVERIVANLTG
MGENI LLINFMASAAKQ---IDP-----KTDVVLFFSLEMRNREIYQRHLMHESQ
MPNEU LLINFLQGAVNDSEKFDK-----NKDVLLFFSLEMRNREIYQRHLMLESQ
. **** . * .
ECOLI VDQTKIRTGQ-LDDEDWARISGTMGILLEKRNIYIDDSSGLTPTEVRSRA
STYPH VDQTRIRTGQ-LDDEDWARISGTMGILLEKRNMYIDDSSGLTPTEVRSRA
HINFL VDQTKIRTGQNLDEIEWNKIASVVGMFKQKNNLFIDDSSGLTPTDVRSRA
BSUBT INAQNLRTGN-LTEEDWGKLTMAMGSL-SNSGIYIDDTPGIRVSEIRAKC
BMOJA INAQNLRTGN-LTEEDWGKLTMAMGSL-SNSGIYIDDTPGIRVSEIRAKC
BSTEA INAQNLRTGK-LTPEDWGKLTMAMGSL-SNAGIYIDDTPSIRVSDIRAKC
TAQUA IDMNRVRLGQ-LTDRDFSRLVDVASRL-SEAPIYIDDTPDLTLMEVRARA
RMARI VDAQAARTGR-LRDEDWRKLARAAGRL-SDAPIFIDDTPSLGVLELRAKC
TMARI VDLYSIRTGY-LDQEKWERLTIAASKL-YKAPIVVDDESLLDPRSLRAKA
SYNEC3 IDSNRLRTGH-FSQAEFEPLTAAMGTL-SSLPIYIDDTASISVTQMRSQV
MLEPR IKLADMRSGR-MTDDDWTRLARRMSEI-SEAPLYIDDSPNLTMMEIRAKA
MTUBE IKLSDMRSGR-MSDDDWTRLARRMSEI-SEAPLFIDDSPNLTMMEIRAKA
SCOEL VALHHMRSGT-MTDDDWTRLARRMPDV-SAAPLYIDDSPNLSMMEIRAKC
RPROW IDSTALRTGM-LSEDKYNRLRKEANTL-SELQFFIDDTPALSISAIRTRA
CTRAC2 VEAKKISVG-DISGRDFQRVVSVVREM-EEHTLLIDDYPGLKITDLRARA
AAEOL VPLFKIRSG-SISNEDLKKLEASAIEL-AKYDIYLDDTPALTTTDLRIRA
BBURG IDSFKVQNSI-LSGQEIKSLNDIINEI-SDSELYIEDTPNISLLTLATQA
TPALL VSATNLRKGL-LQLSDFGRIQNAAGEM-YDAPLYIVDVPNMKLLDLRAVA
HPYLO INMHDLESGR-LDDDQWENLAKCFDHL-SQKKLFFYDKSYVRIEQIRLQL
CPSIT TSCEQLKRG-NFSRDVLSKIESI-GTKLKGTHFFICDNKSTDLNTLIDQA
CTRAC1 ISGEKLQRG-DLSKEELFRVEEA-GETVRESHFYICSDSQYKLNLIANQI
MGENI TSYTL--TNRQRINNVFEELMEA-SSRIKNLPIKLFDYSSLTLQEIRNQI
MPNEU VNYTL--TSRQRLANVYDDLIKA-SEVLRDLPIKIFDYSTITLDEIRAQI
. .
ECOLI RRIAREHGG-I-LIMIDYLQLMRVPALS---DNRTLEIAEISRSLKALAK
STYPH RRIFREHGG-LSLIMIDYLQLMRVPSLS---DNRTLEIAEISRSLKALAK
HINFL RRVYRENGG-LSMIMVDYLQLMRAPAFS---DNRTLEIAEISRSLKALAK
BSUBT RRLKQESG--LGMILIDYLQLIQGSGRSK--DNRQQEV-EISRELKSIAR
BMOJA RRLKQESG--LGMILIDYLQLIQGSGRSK--DNRQQEVSEISRELKSIAR
BSTEA RRLKQESG--LGMIVIDYLQLIQGSGRSK--ENRQQEVSEISRSLKALAR
TAQUA RRLVSQNQ--VGLIIIDYLQLMSGPGSGKSGENRQQEIAAISRGLKALAR
RMARI RRLKAEHD--IGLVIVDYLQLMQASHMPRNAN-REQEIAQISRSLKALAK
TMARI RRMKKEYD--VKAIFVDYLQLMHLKGRK---ESRQQEISEISRSLKLLAR
SYNEC3 RRLQSEQKGPLGMVLIDYLQLMEGG-----SDNRVQELSKITRSLKGLAR
MLEPR RRLRQKTN--LKLVVVDYLQLMTSG---KRFESRQVEVSEFSRHLKLLAK
MTUBE RRLRQKAN--LKLIVVDYLQLMTSG---KKYESRQVEVSEFSRHLKLLAK
SCOEL RRLKQRND--LKLVVIDYLQLMQSGG-SKRAESRQQEVSDMSRNLKLLAK
RPROW RRMKRKHN--LGILFIDYLQLIRGVSKS---ENRVNEISEITQGLKAIAK
CTRAC2 RRMKESYD--IQFLVIDYLQLISSSGNLRNSDSRNQEISEISRMLKNLAR
AAEOL RKLRKEKE--VEFVAVDYLQLLRPPVRK---SSRQEEVAEVSRNLKALAK
BBURG RKLKRFYG--IDIIFVDYISLI---SFETKNLPRHEQVASISKSLKELAR
TPALL RRLCVQEK--IQIIFVDYLGLI---VADNPFAPRYEQFAAISQSLKSLAR
HPYLO RKLKSQHKE-LGIAFIDYLQLMSGS---KATKERHEQIAEISRELKTLAR
CPSIT RELRENQG--IDALFIDYLQLIGSS---KKAENRQNEIAEISRQLRKLAV
CTRAC1 RLLRKEDR--VDVIFIDYLQLINSS---V-GENRQNEIADISRTLRGLAS
MGENI TEVSKTSN--VRLVIIDYLQLVNAL-KNNYGLTRQQEVTMISQSLKAFAK
MPNEU TEVSKTNN--VKLVVIDYLQLVNAF-KNSYNISRQQEVTMISKSLKSFAK
. . .**. *. * . .. *. .*
ECOLI ELNVPVVALSQLNRSLEQR-ADKRPVNSDLRESGS-IEQDADLIMFIYRD
STYPH ELQVPVVALSQLNRSLEQR-ADKRPVNSDLRESGS-IEQDADLIMFIYRD
HINFL ELQVPVVALSQLNRTLEQR-GDKRPVNSDLRESGS-IEQDADLIMFIYRD
BSUBT ELQVPVIALSQLSRGVEQRQ-DKRPMMSDIRESGS-IEQDADIVAFLYRD
BMOJA ELQVPVIALSQLSRGVEQRQ-DKRPMMSDIRESGS-IEQDADIVAFL---
BSTEA ELEVPVIALSQLSRSVEQRQ-DKRPMMSDIRESGS-IEQDADIVAFLYRD
TAQUA ELGIPIIALSQLSRAVEARP-NKRPMLSDLRESGS-IEQDADLVMFIYRD
RMARI ELNVPVVALSQLSRAVETRGGDKRPQLSDLRESGXSIEQDADVVLFIYRP
TMARI ELDIVVIALSQLSRAVEQRE-DKRPRLSDLRESGA-IEQDADTVIFIYRE
SYNEC3 EINAPVIALSQLSRAVESRT-NKRPMMSDLRESGXSIEQDADLIMMIYRD
MLEPR ELELPVVAISQLNRSPEQR-TDKKPMLSDLRESGS-LEQDADVVILLHRP
MTUBE ELEVPVVAISQLNRGPEQR-TDKKPMLADLRESGXSLEQDADVVILLHRP
SCOEL ELEIPVIALSQLNRGPEQR-TDKKPMVSDLRESGS-IEQDADMVILLHRE
RPROW ELNIPVIALSQLSRAVELRE-DKKPMLSDLRESGT-IEQDADIVMFIYRE
CTRAC2 ELNIPILCLSQLSRKVEDR-ANHRPLMSDLRESGS-IEQDADQIMFLLRR
AAEOL ELHIPVMALAQLSREVEKR-SDKRPQLADLRESGQ-IEQDADLILFLHRP
BBURG ELEIPIVALSQLTRDTEGRE----PNLASLRESGA-LEQDADIVILLHRD
TPALL ELDIPIVALSQVGRPAEGSA----PNLADIRGSGA-IEQDADVVMFLHRD
HPYLO ELEIPIIALVQLNRSLENRD-DKRPILSDIKDSGG-IEQDADIVLFLYRG
CPSIT ELQIPVVCLSQLSRKVEDR-GDKRPMLSDLRDSGQ-IEQDADVILFLHRK
CTRAC1 ELNIPIVCLSQLSRKVEDR-ANKVP-------------------------
MGENI EFNTPIIAAAQLSRRIEERK-DSRPILSDLRESGS-IEQDADMVLFIHRT
MPNEU EFNVPIIAAAQLSRRIEERK-DSRPILSDLRESGS-IEQDADMVLFIHRV
*. .. *. * * *
ECOLI EVYHENSDLK--------G--------------------------IAEII
STYPH EVYHENSDLK--------G--------------------------IAEII
HINFL EVYNDNSEDK--------G--------------------------VAEII
BSUBT DYY--DKETENKN--------------------------------IIEII
BMOJA --------------------------------------------------
BSTEA DYY--NKDSENKN--------------------------------IIEII
TAQUA EYY--NPHSEKAG--------------------------------IAEII
RMARI ERY--GITVDENGN-PTEG--------------------------IAEII
TMARI EYYRSKKSKEESKL-HEPH--------------------------EAEII
SYNEC3 EYY-------NPDT-PDPG--------------------------VAELL
MLEPR DAF-------DRDD-PRGG--------------------------EADLI
MTUBE DAF-------DRDD-PRGG--------------------------EADFI
SCOEL DAY-------EKES-PRAG--------------------------EADLI
RPROW EYYLTRKEPVAGDA-KHAEWLDKLNKVY----------------NIADII
CTRAC2 EYYDPNDKPGT-----------------------------------AELI
AAEOL EYYKKKPNPEEQG--------------------------------IAEVI
BBURG KDFKFESSAEIEPI-E------------------------------TKVI
TPALL RN----------ET-E------------------------------TQLI
HPYLO YIYQMRAEDNKIDKLKKEGKIEEAQELYLKVNEERRIHKQNGSIEEAEII
CPSIT DYYS-----------------------------------QEATKGLSEII
CTRAC1 --------------------------------------------------
MGENI N----DDKKEQEEE--NTNLFE------------------------VELI
MPNEU K----DEDSENQEAIGHNNIFQ------------------------VELI
ECOLI IGKQRNGPIGTVRLTFNGQWSRFDNYAG--P-QYDDE-------------
STYPH IGKQRNGPIGTVRLTFNGQWSRFDNYAG--P-QYDDE-------------
HINFL IGKQRNGPIGRVRLKFNGQFSRFDNLAE--QREYRDD-------------
BSUBT IAKQRNGPVGTVSLAFVKEYNKFVNL----ERRF------------DDAG
BMOJA --------------------------------------------------
BSTEA IAKQRNGPVGTVQLAFIKEYNKFVNL----ERRF------------DEAQ
TAQUA VGKQRNGPTGTVELQFHASHVRFNDL----AR------------------
RMARI IGKQRNGPTGTVRLAFINQYARFENL----TM-YQPEPGTPLPETPDETI
TMARI IGKQRNGPVGTITLIFDPRTVTFHEV----D------------------V
SYNEC3 IVKHRNGPTGVVKLLFKPEFTQFLNL----QRSND---------------
MLEPR LAKHRNGPTKTVTVAHQLHLSRFTNMAR----------------------
MTUBE LAKHRNGPTKTVTVAHQLHLSRFANMAR----------------------
SCOEL VAKHRNGPTATITVAFQGHYSRFVDMAQ--T-------------------
RPROW IAKHRNGPVGNVSLYYDSQFSKFGNLEK---RTFNS--------------
CTRAC2 VAKNRHGSIGSVQLVFEKDFARF--------RNY---AGCEFPG------
AAEOL IAKQRQGPTDIVKLAFIKEYTKFANLEALPEQPPEEEELSEIIETQEDEG
BBURG VAKHRNGPTGRADILFLPHITKFVN-------------------------
TPALL LAKQRNGPIGTVELEFQASFTRFVC-------------------------
HPYLO VAKNRNGATGTVYTRFNAPFTRYEDM--------------PIDSHLEEGQ
CPSIT VGKNRHGSVFSTTLRFNSCTGKFT--------------------------
CTRAC1 --------------CFQIC-------------------------------
MGENI LEKNRNGPNGKVKLNFRSDTSSF-------ISQYSP--------------
MPNEU LEKNRSGPTGKVQFDFQSDVSTF------RVREYNP--------------
ECOLI -----------
STYPH -----------
HINFL ----------Y
BSUBT VPPGA------
BMOJA -----------
BSTEA IPPGA------
TAQUA ---DA------
RMARI LPSGPPDEAPF
TMARI VHS--------
SYNEC3 ----------Y
MLEPR -----------
MTUBE -----------
SCOEL -----------
RPROW ----------I
CTRAC2 -----------
AAEOL F-----EDIDF
BBURG ------KDHQY
TPALL ------KSP--
HPYLO ETKVDYDIVTT
CPSIT -----IQKEAW
CTRAC1 ------ETAVK
MGENI ----SFDDQYS
MPNEU -------DGYS
About the alignment.
There were 22 identical residues shared by the first 22 available bacterial DnaB-like helicase sequences. There would be nine more if C. trachomatis and B. mojavensis had not terminated so prematurely.
Phe59 is the only identical residue found in the DnaB N-terminal domain. Its side chain is fully exposed to solvent according to the crystal.
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